Objective:To explore effects of early intervention on physical and neurobehavioral development of premature infants during infant period. Methods:One hundred and tweenty-six premature infants were randomly divided into intervention group (n = 63) and control one (n = 63). They were treated with early intervention in intervention group,while a routine physical examination was given to control group. The physique,mood,sleep and intellectual development indexes were observed and recorded regularly,and then compared between two groups. Results:The measured values of weight,height,and head circumference were significantly greater in the intervention group(P < 0.01). At corrected 3 months old,the sleep conditions were significantly better in the intervention group(P < 0.05). The number of crying in intervention group was significantly less during the physical examination process of the corrected 6 months (P < 0.05). The developmental quotients in intervention group were remarkably increased,when they were corrected 6 and 12 months old (P < 0.01). Conclusion:Early intervention is beneficial for the physical development of premature infants,and is helpfur for reduce of sleep problems,negative emotion of crying,and improvement of intelligence development of premature infants.