Objective:To determine the expression of HOTAIR in common renal carcinoma and normal renal cell lines and establish a renal carcinoma cell line that highly expresses HOTAIR. Methods:①q-PCR was used to detect the expression of HOTAIR in four renal carcinoma cell lines (os-rc-2,kevt-3,achn and 769-p) and a normal renal cell line(HK-2). ②Infection with lentiviral (pLenti GFP Puro-HOTAIR) carrying HOTAIR gene in 769-p cell line and puromycin-resistance screening were performed to establish the cell line that highly expresses HOTAIR. Results:① HOTAIR was expressed in five cell lines (from high to low):kevt-3,os-rc-2,achn,769-p,and HK-2. ②HOTAIR was high expressed in 769-p after infected with lentiviral (pLenti GFP Puro-HOTAIR). Conclusion:①HOTAIR expression in high metastatic carcinoma cell lines(kevt-3,os-rc-2) was higher than that in low metastatic renal carcinoma cell lines (achn,769-p ) and normal renal cell line(HK-2).②The 769-p-HOTAIR cell line stably expressed HOTAIR.