Objective:To investigate the major effects of environmental endocrine disruptors (EDCs) BPA on SD rat blood-brain barrier component expression,cell viability of astrocytes and explore the mechanism. Methods:The MTT assay was performed to evaluate the astrocyte viability,HPLC was performed to detect glutamate levels,and by real-time quantitative PCR the mRNA levels of Claudin-3,Claudin-4,Claudin-5,JAM-1,JAM-2,ZO-1 and E-cadherin were detected. Results:After astrocytes were exposed to BPA for 24 or 48 hours,the cell viability significantly decreased. The realtime-PCR data showed that mRNA levels of blood-brain barrier tight junctional protein,Claudin-3,Claudin-4,JAM-1,E-cadherin,were significantly down-regulated. Meanwhile,Claudin-5,JAM-2 and ZO-1 mRNA expressions were significantly up-regulated. Furthermore,HPLC showed that the concentration of glutamate was increased. Conclusion:Taken together,EDCs BPA demonstrated the potential toxic effects on the blood-brain barrier integrity.