Objective:To study the biological characteristics of cytotoxic T lymphocyte (CTL) originated from dendritic cells (DC) loaded with the Epstein-Birr virus latent membrane proteins (LMPs),and to detect the cytotoxic effect of LMPs-CTL on the LMPs positive nasopharyngeal carcinoma cell SUNE. Methods:The mononuclear cells were isolated from human peripheral blood by lymphocyte separation medium. The adherent method was performed. Cytokines of IL-4 and GM-CSF were performed to induce mature DC. Loaded with LMPs polypeptide antigen,the mature DC presented antigen to autologous T lymphocyte for preparation of LMPs-CTL. The cytotoxic effect of LMPs-CTL on SUNE cells was detected by the CCK8 method. The secretion of interferon-γ (IFN-γ) of LMPs-CTL and cell proliferation was detected by ELISA and carboxy fluoroscein succinimidyl ester (CFSE),respectively. Results:The killing efficiency of LMPs-CTL for SUNE was (43.47 ± 1.93)% in 12h and (77.15 ± 3.18)% in 24 h,which were higher than those in the control group[(11.45 ± 3.06)% and (24.27 ± 13.2)% (P < 0.05)]. The ability of LMPs-CTL cell proliferation simulated with SUNE cells was stronger than that in the control group. The IFN-γ secretion level secreted by LMPs-CTL was (613.40 ± 121.77) pg/ml,which was significantly higher than that secreted by the control group (86.90 ± 3.70) pg/ml (P < 0.05). Conclusion:LMPs specific CTL can be successfully induced by mature DC and possess preferable specific killing activity on LMPs positive nasopharyngeal carcinoma cells.