Objective:To analyze the polymorphism distributions of 19 STR loci including D21S11,D6S1043,Penta E,PGA,D2S1338,D11S51,D19S433,D12S391,CSF1PO,D3S1358,Penta D,VWA,D13S317,THO1,TPOX,D8S1179,D16S539,D5S818 and D7S820 in Jiangsu population,and calculate their gene frequency(P),power of discrimination(DP),unbiased expected heterozygosity(H),polymorphism information content(PIC),and probability of paternity exclusion(PE). Methods:Capillary electrophoresis was detected using PCR via 3130 sequencing. Analysis software was used to analyze data. Results:The DP value and PE value of Penta E were the highest in the 19 STR sites. The cumulate PE of the 19 STR loci was above 0.9999. Conclusion:Objective allelic polymorphism data were obtained from a large number of sample experiments and allele frequency,which provide an objective basis for identification in disputed paternity of Jiangsu area.