Objective:To observe the anti-cancer activity of McAb NJ001 against lung adenocarcinoma cells,and explore its molecular mechanism. Methods:We selected SPC-A1 cell lines as research object,used flow cytometry to detect cell apoptosis,and observed morphological changes of SPC-A1 cells by phase contrast microscope. We detected the expressions of apoptosis-related proteins such as caspase-8,caspase-9,caspase-3,PARP,Bax,and Bcl-2 by Western blotting assays. Results:McAb NJ001 induced apoptosis in SPC-A1 cells in a dose-dependent manner. After treatment with NJ001,SPC-A1 cells showed shrinkage,and became round or even fell off,which were typical morphological changes of apoptosis. Results:Western blots showed that after treatment with McAb NJ001,PAPR were cleaved so that caspase-3 expression was decreased,caspase-8 and caspase-9 were activated and their protein expression levels were increased. The expression of pro-apoptotic protein Bax increased while the expression of anti-apoptotic protein Bcl-2 decreased. Conclusion:McAb NJ001 has strong anti-cancer activity due to its apoptosis inducing effect. In addition,caspase activation and up-regulation of Bax/Bcl-2 were involved in NJ001-induced apoptosis.