Changes of rat islet function and mammalian target of rapamycin pathway related protein expression under chronic intermittent hypoxia
Author:
  • Article
  • | |
  • Metrics
  • | | | | |
  • Comments
    Abstract:

    Objective:To investigate the effect and possible mechanism of mammalian target of rapamycin (mTOR) protein and its related pathway on islet β cell proliferation in rats with chronic intermittent hypoxia (CIH). Methods:Twenty-four adult male Sprague-Dawley (SD) rats were randomly divided into two groups (the CIH group and the control group). The CIH group was fed in low oxygen cabins (60 s/N2 and 60 s/air alternately,oxygen concentrations between 5% and 21%,between 8:30~16:30 each day) for 35 days. We detected blood glucose and serum insulin levels by ELISA and calculated the insulin resistance index (HOMA-IR) and β cell insulin secretory function (HOMA-β) of the two groups. Glucose tolerance was evaluated by oral glucose tolerance test. The proliferation of pancreatic β cells was tested by immunofluorescence assay and mTOR and related pathway protein expressions were tested by Western blotting assays. Results:The fasting blood glucose,serum insulin levels and insulin resistance index increased (P < 0.01),while glucose tolerance and pancreatic β-cell function decreased (P < 0.05),in comparison with the control group. The number of ki67 positive β cells increased about five-fold while the mTOR related pathway protein expression of pancreatic tissues was activated. Conclusion:The rats in CIH condition showed insulin resistance,glucose tolerance impairment and pancreatic β-cell dysfunction;meanwhile,the activated mTOR pathway may play an important role in pancreatic β-cell proliferation.

    Reference
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

裴 静,姚 蒙,焦 成,张敏娟,殷 敏,程 雷.慢性间歇性缺氧条件下大鼠胰岛功能与mTOR通路相关蛋白表达的变化[J].南京医科大学学报(自然科学版英文版),2014,(7):915-920.

Copy
Share
Article Metrics
  • Abstract:2357
  • PDF: 2022
  • HTML: 59
  • Cited by: 0
History
  • Received:March 01,2014
  • Online: July 05,2014
Article QR Code