Objective:To construct recombinant adenovirus carrying mouse PPA1 gene,and detect its expression in mouse islets and β－TC6 cells,and observe its effect on fatty acid－induced pancreatic β cell apoptosis. Methods:The target gene of PPA1 was cloned into the shuttle vector pAdtracd－CMV. After identified by PCR,the plasmid was linearized by PmeⅠ and recombined with backbone pAdeasy－1 in BJ5183 bacteria. The recombinant plasmid was linearized by PacⅠ enzymes digestion,transfected into QBI－293A cells,and packed for Ad－PPA1 adenovirus. Accoring to the green fluorescent protein (GFP),the titer and infection rate was detected. The mouse islets and β－TC6 cells were infected by recombinant adenovirus and the protein level of PPA1 was detected by Western blot. By Hoechst staining,the effect of PPA1 on cell apoptosis was detected. Results:The recombinant adenovirus vector pAdeasy－PPA1 was successfully constructed. After pAdeasy－PPA1 was transfected into QBI－293A cells,cytopathic effect showed that the adenovirus was successfully packed. The recombinant adenovirus containing mouse PPA1 gene effectively infected mouse islets and β－TC6 cells,and over－express the protein levels of PPA1. Hoechst staining showed that over－expression of PPA1 protected β－TC6 cells against fatty acid－induced apoptosis. Conclusion:The recombinant adenovirus expressing PPA1 gene has been successfully obtained and over－expression of PPA1 has anti－apoptosis effect,which provided a basis for further study of PPA1 on pancreatic β－cell function.