Objective:To construct a hITF gene delivery system,and assess its gene transfection efficiency. Methods:ITF eukaryotic expression vector was constructed. Chitosan nanoparticles were prepared by a complex coacervation method,and its size and morphology were assessed using transmission electron microscope (TEM). Gene transfer capability of nanoparticles was assessed in HEK293 cells. The transcription and expression of hITF were determined by RT－PCR and Western blot. Results:hITF eukaryotic expression vector was successfully constructed. The chitosan nanoparticles were prepared by complex coacervation method with different N/P ratio. Nanoparticle size less than 1 000 nm and narrow distribution of nanoparticle diameter were observed by TEM. The transfection efficiency assessed by fluorescence microscopy and flow cytometry was about 80%,similar to the transfection efficiency of Lipofectamine. RT－PCR and Western blot assay demonstrated that hITF was transfected and expressed successfully. Conclusion:An optimal hITF gene delivery system was successfully constructed. This research laid foundations for further application of hITF gene therapy.