Objective:To analyze the possible association between VDAC2 gene polymorphism and sperm quality of idiopathic male infertility. Methods:A genetic typing of VDAC2 gene locuses (rs2804535,rs7896741,rs11001334 and rs1259503) by TaqMan SNP genotyping technique and a semen analysis by computer－assisted semen analysis (CASA) were respectively performed in 523 Han－Chinese males with idiopathic infertility in order to analyze the correlation between gene polymorphism and sperm quality. Results:No significant association between genotypes and semen quality was found at locuses rs2804535 and rs7896741. However,at rs 11001334,it was found that the TT genotype showed a significant lower sperm concentration compared with the CC genotype (P = 0.045). Moreover,compare with CC genotype,the sperm motility of CT genotype had a significant increase (P = 0.026);meanwhile,the same trend was found in the combination of CT and TT genotypes (P = 0.037). However,at rs1259503,the combination of GC and CC genotypes had a remarkable reduction of semen volume than GG genotype (P = 0.039). Conclusion:These findings indicate that the gene polymorphism in the VDAC2 gene may be associated with semen quality.