Objective:To validate the effect of hypertension on small conductance calcium-activated potassium channels in cavernous tissue of spontaneously hypertensive rats. Methods: Male spontaneously hypertensive rats (SHR) (n = 8) and normotensive Wistar Kyoto rats (WKY) (n = 8) were selected. A series of electric stimulation were utilized to the corpus cavernosum nerves of rats, the changes of ICP /MAP were observed repeatedly, and the expressions of SKCa protein and mRNA were assessed by Western blotting and RT-PCR. Results: Compared with the WKY group, ICP /MAP and the expression of SKCa gene mRNA and protein were significantly lower in the SHR group (P < 0.05), and the expression of SKCa protein and mRNA was significantly lower in the SHR group in comparison with that of the WKY group (P < 0.05). Conclusion: Erectile dysfunction in hypertension is associated with the low expression of SKCa in the cavernous tissue of spontaneously hypertensive rats.