Objective:To study the difference of expression of miRNAs of inflammatory cytokines in lipoplysaccharide(LPS)-stimulated mast cell secretion. Methods:P815 cells were stimulated by LPS (1 μg/ml) for 16 hours. The expression levels of TNF-α and IL-6 in supernatant were detected by ELISA. Real-time PCR was performed to detect the expression differcence of miRNAs after the cells were recollected. Results:In P815 after LPS stimulation,the secretions of TNF-α and IL-6 were largely up-regulated than those of control (P < 0.05). Five miRNAs in the intervention group were found increasingly expressed compared with those in the control group,with miRNA-126 1.3-fold,miRNA-155 1.2-fold,miRNA-223 1.7-fold,miRNA-221 1.2-fold,and miRNA-192 1.4-fold (P < 0.05). Conclusion: miRNAs were expressed differentially in mast cells after LPS stimulation. The differcent expressions of miRNA may be invovled in mast cells function changes.