Objective:To construct a recombinant adenovirus that express mouse Ets-1 in primary cultured mouse islets. Methods:To generate pAdTrack-CMV-Ets-1 shuttle vector,the CDS region of Ets-1 was amplified by PCR,and then purified and cloned into the pAdTrack-CMV. pAdTrack-CMV-Ets-1 was recombined with back-bone pAdEasy-1 in BJ5183 bacteria. The resulting vector was transfected into QBI-293A cells to generate recombinant adenovirus. After been amplified and purified,the recombinant adenovirus were used to infect primary cultured mouse islets and INS-1 cells. The protein levels of Ets-1 were determined by Western blotting assay. Results:The Adv-Ets-1 was established successfully and proved to be high infective and possess a high expression potential. Conclusion:The Ets-1 recombinant adenovirus was successfully constructed,which provided a foundation for further study of the function of Ets-1 in primary islets.