Objective:To design and compound of twin drug with nNOS-PSD-95 interrupts and gabapentin,pregebalin,and to find new drugs for the treatment of neuropathic pain. Methods:The nNOS-PSD-95 interrupts and gabapentin,pregebalin were connected to form amide. The analgesic effects of target compounds were evaluated by neuropathic pain model. Results:All target compounds showed different degree analgesic effects in neuropathic pain, the analgesic effect of twin drug of nNOS-PSD-95 interrupts and gabapentin,pregabalin twin drug in neuropathic pain was stronger than that of single target drugs. Conclusion:Twin drug with nNOS-PSD-95 interrupts and gabapentin,pregebalin may get a better analgesic effect than that of single target drugs in neuropathic pain.