Objective:To prepare doxorubicin liposomals modified with PEG and cRGD,and study physicochemical properties of liposomals along with targeting effects on Hela cells. Methods:Using pH gradient method to prepare the liposomes modified with cRGD. The characteristics were observed with TEM and UV spectrophotometer. The targeting effect was determined by immunofluorescence and flow cytometry on Hela cells,and cell apoptosis was measured by MTT assay and flow cytometry. Results:The particle size of liposomes was between 100-180 nm,and encapsulation ratio detected by UV spectrophotometer was 72%. The result of immunofluorescence and flow cytometry showed targeting effect of liposomes on Hela cells,which was significantly better than that of control group. Conclusion:These doxorubicin liposomes with high encapsulation ratio and small particle size exhibited an effect of cell targeting and cell penetrating,which might be a novel approach for specific and efficient intracellular drug delivery.