Objective:To investigate relationship between expression of MUC4 and MUC16 and clinical pathological characteristics in ovarian cancer cell line SKOV-3,OVAR-3,CAOV-3 and epithelial ovarian cancer(EOC) tissues. Methods:①Ovarian cell line SKOV-3,OVAR-3 and CAOV-3 were cultured,and then immunocytochemistry and Western Blot analysis were taken to detect the protein expression of MUC4 and MUC16;②Ovarian normal and epithelial cancer tissues were collected. IHC was used to detect the protein expression of MUC4 and MUC16 in ovarian normal and cancer tissues. Results:①The immunocytochemistry and Western blot analysis showed that the expression of MUC4 protein was not detected in SKOV-3 and OVCAR-3 cell lines,but it was observed in CAOV-3. While,the expression of MUC16 protein was detected in three ovarian cancer cell lines. Moreover,the level of MUC16 was highest in OVCAR-3 cell line,second in CAOV-3 and the lowest in SKOV-3. The differences were statistically significant(P < 0.05);②The positive expression of MUC4 and MUC16 was low in normal ovarian tissue and benign ovarian tumor,but was high in borderline ovarian tumor and EOC;③the positive expression rate of MUC4 and MUC16 among different subtypes including serous tumor(86.36%,75%);mucinous tumor was(75%,25%) respectively. The positive expression rate of MUC4 and MUC16 at different tumor stages was including early stage(91.67%,50%) and advanced stage (75%,85.7%). Conclusion:① There were different expressions of MUC4 and MUC16 in ovarian epithelial cancer cell lines;② MUC4 alone or together with MUC16 may be as a good diagnosis and prognosis marker for EOC.