Objective:To explore the role of Oatp3 on the PFOS-induced Sertoli cell injury. Methods:The primary Sertoli cell was isolated from 20 day old male SD rat. Cytotoxicity assay,RNAi experiments and immunoblotting analysis were conduced to explore the role of Oatp3 on the PFOS-induced Sertoli cell injury. Results:There is no significant toxic effects observed after PFOS treatment with the dose range from 0~30 μM for 24 hrs(P > 0.05). In addition,compared with the control group,40 μmol/L of PFOS significantly decreased the survival rate of Sertoli cells(P < 0.01).The IC50 of PFOS on Sertoli cell is 45.6 μmol/L. Further more,40 μmol/L of PFOS significantly increased the expressions of Oatp3 on cells,which was inhibited by Oatp3 siRNA(P < 0.01). Compared with Mock group,40 μmol/L of PFOS significantly decreased the survival rate of Sertoli cells(P < 0.01),which was inhibited by Oatp3 siRNA(P < 0.01). Conclusion:Oatp3 is involved in PFOS-induced Sertoli cell injury.