Objective:This study investigated the expression and significance of both P-cadherin and HSP27 proteins in gastric carcinoma tissues as well as the relationships among the clinical pathologic factors, and discussed the meaning of being prognostic indicators. Methods: High throughput technology of tissue microarray and immunohistochemical SP method was employed to investigate the expression of P-cadherin and HSP27 proteins in 75 gastric carcinoma specimens from patients without chemo-radiation therapy and 75 non-tumor gastric tissues. Survival analysis was carried out on the follow-up of patients. Results: In gastric cancer tissue and normal gastric tissue, the expression rates of P-cadherin, HSP27 were detected to be 40.0% and 82.7%, 64.0% and 30.7%, respectively (both P < 0.001). High expression of P-cadherin and low expression of HSP27 were related to clinical stage, differentiation degree and lymph node metastasis (P < 0.05), the high expression of HSP27 was also correlated with tumor size (P <0.05). According to the results of Pearson correlation analysis, the expression rates of P-cadherin and HSP27 in gastric cancer were negatively correlated to each other (P < 0.05). Kaplan-Meier analyses showed that P-cadherin and HSP27 were significantly associated with the prognosis (P < 0.05). The results of univariate analysis of Cox showed that P-cadherin, HSP27, clinical stage, tumor size and lymph node metastasis were significantly correlated with the prognosis (P < 0.05); The multivariate analysis of Cox model indicated that HSP27 and clinical stage were significantly correlated with the prognosis (P < 0.05). Conclusion: The low expression of P-cadherin in gastric carcinoma and high expression of HSP27, which was closely related with the clinical pathological features and biological behaviors of gastric cancer. Expression of P-cadherin and HSP27 were negatively correlated in gastric carcinoma. P-cadherin and HSP27 could be used as the candidate index to judge the prognosis of patients with gastric carcinoma.