Objective:To prepare a fibronectin (FN) modified poly(lactic-co-glycolic acid) (PLGA) membrane via dopamine (DP), and evaluate the effects of FN on cell adhesion of ME3T3-E1. Methods: Attenuated total reflectance Fourier transform infrared spectrometer (FTIR), contact angle meter and quartz crystal microbalance with dissipation were performed to measure the surface groups, contact angle and FN loading of the samples; Time lapse imaging system was performed to observe cell adhesion of ME3T3-E1 every 3 min, and cell counting Kit-8 was used to measure the cell adhesion 0.5, 1.0, 4.0, 8.0 h after cell vaccination in phosphate buffered saline (PBS). Results: FTIR and contact angle showed DP was successfully grafted onto PLGA, and improved hydrophilicity was acquired on DP modified PLGA surfaces. Quartz crystal microbalance with dissipation showed that FN loading was 22.5 ng/cm2. Higher cell adhesion ratio was observed with time lapse imaging system and CCK-8 tests in FN loading samples than in PLGA and PLGA-DP membranes (P < 0.05). Conclusions: MC3T3-E1 can adhere to FN modified PLGA membranes via DP in PBS successfully.