Objective:To investigate effects of human anti-Trop-2 Fab antibody on biological characteristics of cervical cancer cells. Methods:The expression of Trop-2 protein from cervical cancer cells was assessed by flow cytometry(FCM)and immunofluores- cence. The effect of human anti-Trop-2 Fab antibody on migration in human cervical cells was examined by wound healing assay and transwell test. Cell apoptosis induced by Trop-2 Fab was analyzed by flow cytometry. Human anti-Trop-2 Fab antibody influence on cervical cell proliferation was detected by cell counting kit-8 (CCK8)assay. Results:Trop-2 protein was confirmed to express on human cervical cells. Human anti-Trop-2 Fab antibody inhibited human cervical cancer cell growth,migration and invasion,at the same time induced cervical cancer cell apoptosis. Conclusion:Human anti-Trop-2 Fab antibody can significantly inhibit the malignant biological behavior of cervical cancer cells. This fusion antibody may play a role in tumor targeting therapy,and Trop-2 may be a potential therapeutic target for cervical cancer.