Objective:We sought to determine whether single nucleotide polymorphisms(SNPs) in toll-like receptor(TLR) 2 subfamily genes affect the risk of asthma in adults from southeastern China,and the association between SNPs and asthma clinical phenotypes. Methods:A total of 318 asthmatic patients and 352 non-asthmatic controls were recruited. Eight SNPs in TLR2 subfamily genes were detected using Genome Lab SNP stream. Each SNP and haploid type as well as asthma phenotype were analyzed. Results:We found that patients with the TLR2/rs7656411 TT variant homozygote had a significantly reduced risk of asthma when compared with those with the GG wild-type homozygote[adjusted odds ratio (OR):0.63; 95% confidence interval (CI):0.41-0.98; P = 0.41]. There was no significant difference between rs7656411TT+GT and asthma (OR:0.77; 95% CI:0.54-1.09; P≥ 0.05). Furthermore,a positive association was observed between the T allele of rs2381289 in TLR6 and allergic rhinitis in asthma(OR:1.79; 95% CI:1.10-2.91; P = 0.025),while the A allele of rs11466651 in TLR10 was negatively associated with allergic rhinitis(OR:0.49; 95%CI:0.26-0.95; P = 0.046). Conclusion:Our results indicate that a genetic variant in the TLR2 subfamily may play a role in susceptibility to asthma.