Objective:To discuss the effects of RNA interference silencing MKK7 on SH SY5Y cells apoptosis. Methods:Three articles MKK7 siRNA(MKK7siRNA-1,MKK7siRNA-2 and MKK7siRNA-3)were designed and synthesized,the negative control siRNA with red fluorescence,which had no homology to the MKK7 gene,was transfected into SH SY5Y cells with the lipofectamine 2000 and the transfection effect was tested using fluorescence microscope,the MKK7 was detected by RT-PCR and Western blot, then were screened MKK7siRNAs. After the MKK7siRNAs were transfected into SH-SY5Y cells,the cell vitality was detested using CCK-8,and the apoptosis rate was tested by Hochest 33258 dyeing and flow cytometry instrument,the expression of p-JNK and the cleaved caspase-3 protein was detected by western blot. Results:①MKK7siRNA-3 has lowest MKK7 mRNA and protein expression;② compared with the control group,MKK7siRNA-3 group has no statistical difference in cell viability,lower p-JNK and cleaved caspase-3 expression and apoptosis rate. Conclusion: The RNA interference silencing MKK7 can down-regulate the JNK phosphorylation ,which forther inhibit the SH SY5Y cell apoptosis.