Objective:To study the association between polymorphisms in H19 gene,which transcribes a long non-coding RNA,and susceptibility to premature coronary artery disease (pCAD). Methods: Four polymorphisms(rs2067051,rs2251375,rs217727,rs4929984) of H19 gene were analyzed in 213 pCAD patients and 776 control subjects. Polymorphisms were genotyped by TaqMan technology. The statistical analysis was implemented in SPSS. Results: The frequencies of genotype CT,TT and allele T in pCAD group of rs217727 were higher than that in the control group. Multivariate logistic regression analysis showed that the rs217727 polymorhpism of H19 gene was independently associated with the occurrence of pCAD (P < 0.01). The risk of patients with T allele and TT homozygous genotype was 2.42 times(OR=2.42,95%CI=1.56~3.71)and 3.01 times(OR=3.01,95%CI=1.87~4.85)than that of patients with CC homozygous genotype. Conclusion: H19 gene rs217727 polymorphism is associated with the susceptibility of pCAD,and T allele may be a genetic susceptibility factor of pCAD.