To construct a full human anti-toll-like receptor 4 (TLR4)IgG expression vector,and to express and purify it in 293 free style cells,as well as analyze the biological activity of anti-TLR4 antibody. Methods:We designed a primer for amplify mAb variable region. VH and VL gene were cloned into pFUSE-CHIg-hG1 and pFUSE-CLIg-hl expression vectors,respectively,and both transfected into 293 freestyle cells. The IgG was purified by protein A column and the immune specificity of the mAb was detected by enzyme-linked immunosorbent assay(ELISA),Western blotting assay,Co-IP,mass spectrometry(MS)and Biacore. Then,the interaction of this antibody with TNF-a expression in THP1 cell was detected. Results:The results demonstrated that the full human anti-TLR4 IgG was successfully produced. This mAb effectively recognized TLR4 protein and inhibited the expression of THF-a in THP1 cells with the inhibition rate of 85.7%. Conclusion:The reconstructive full human anti-TLR4 IgG could recognize TLR4 protein and has obvious neutralizing effect,and may be potentially utilized for inflammation therapy.