Objective: To identify the potential biological function of hsa-miR-363. Methods: Firstly we analyzed hsa-miR-363 base sequence,chromosomalposition and conservation of the sequence through miRbase and UCSC databases. Secondly, we predicted the target genes by the miRNA databases, including Miranda, miRDB, PicTar, and TargetScan. Then the intersection of these predicted genes were done for further study. Finally, we clarified those cell functions and signaling pathways regulated by hsa-miR-363 target genes using GO and KEGG pathway analysis. Results: According to these bioinformatics data, we found that hsa-miR-363 might have a wide range of functions, and some potential target genes of this microRNA participated in the initiation and development of endometriosis. Conclusion: Hsa-miR-363 might be related to the pathogenesis of endometriosis, and it may provide a potential target for the clinical therapy to endometriosis.