Objective:To explore the regulation of angiogenic gene AGGF1 expression by miR-372. Methods: Bioinformatic prediction was performed to find the candidate microRNAs that may target AGGF1. Luciferase assays using AGGF1 3′-untranslational region (3′-UTR) reporters were performed to verify the binding of miR-372 to the AGGF1 3′-UTR; Real-time quantitative PCR (qPCR) and Western Blot assays were performed to measure the endogenous AGGF1 expression in 293T cells and HUVEC cells at mRNA and protein levels affected by overexpression of miR-372. In vitro angiogenesis assay was performed to verify the effects of miR-372 mediated downregulation of AGGF1 on angiogenesis. Results: Bioinformatic assays indicated that miR-372 targeted AGGF1. Luciferase assays confirmed that miR-372 downregulated AGGF1 expression directly through the miR-372 binding sites on 3′-UTR of AGGF1. qPCR and Western Blot assays revealed overexpression of miR-372 by mimic reduced AGGF1 expression at both mRNA and protein levels. In vitro matrigel based angiogenesis assay showed that overexpression of miR-372 reduced angiogenesis by downregulation of AGGF1. Conclusion: miR-372 downregulates AGGF1 expression and reduces angiogenesis.