Objective:To detect the expression of miR-145 in non-small cell lung cancer(NSCLC) and explore it’s regulatory effect on N-cadherin in the invasion and metastasis of NSCLC. Methods:Real-Time PCR was performed to detect the expression of miR-145 and N-cadherin in 31 NSCLC tumor tissues. MiR-145 mimics was transiently transfected into SPC-A1 cells by lipofectamine method. RT-PCR and Western blot were used to detect the expression of N-cadherin. The invasion activity was detected using transwell assay. Results:MiR-145 expression was reduced significantly and N-cadherin expression was increased significantly in NSCLC tumor tissues,respectively. In addition,the expressions of miR-145 and N-cadherin were both closely correlated with lymph node metastasis. MiR-145 could significantly downregulate the expression of N-cadherin after transfection of mimics in SPC-A1 cells,and suppress invasion ability of cells. Conclusion:MiR-145 is downregulated in NSCLC,and may regulate the invasion and metastasis of NSCLC through targeting N-cadherin.