Objective:To investigate the expression of miR-30e* and the role of miR-30e* in cisplatin induced mouse proximal tubular cells (mPTCs) apoptosis and mitochondrial injury. Methods:Expression of miR-30e* in mPTCs treated with cisplatin were determined by quantitative real-time PCR (qRT-PCR). To stably over expression or knock down miR-30e*, recombinant lentiviral expressing vectors were used for transfection of mPTCs. Flow cytometry was performed to analyze the apoptosis of mPTCs after transfection and treated with or without cisplatin. Mitochondrial DNA (mtDNA) copy numbers were determined by qRT-PCR and mitochondrial membrane potential was examined by JC-1 staining. Results:Cisplatin reduced the expression levels of miR-30e* in a dose and time-dependent manner. MPTCs transfected with over expression miR-30e* recombinant lentiviral expressing vector gained an increased expression of miR-30e* for seven fold. While knock down of miR-30e* decreased the expression of miR-30e* for 50% compared with vehicle. The apoptosis of mPTCs increased when treated with cisplatin,over expression miR-30e* reduced cisplatin induced apoptosis and knock down of miR-30e* facilitated cisplatin induced apoptosis. mtDNA significantly decreased after cisplatin treatment for 48 h,later than the decrease expression of miR-30e*. JC-1 staining revealed ectopic miR-30e* can protect mitochondrial membrane from cisplatin induced injury. Conclusion:Cisplatin reduced the expression levels of miR-30e* in vitro. Ectopic miR-30e* can protect mPTCs from apoptosis and mitochondrial membrane potential changes induced by cisplatin.