Objective:To investigate the effects and mechanism of BRD4 inhibitor JQ1 on the growth of human non-small cell lung cancer (NSCLC)cells including erlotinib resistant cells. Methods:The inhibitory effects of JQ1 on the growth of human NSCLC were observed at different concentrations for 72 hours by sulforhodamine B (SRB)assay. mRNA and protein levels of eIF4E were detected by quantitative real-time PCR assay and Western blot,respectively. Results:JQ1 inhibited the growth of NSCLC cells including erlotinib parental and resistant cells in a dose dependent manner. The mRNA and protein expression levels of eIF4E decreased significantly by JQ1 treatment. Conclusion:JQ1 inhibits the growth of NSCLC cells possibly through downregulating eIF4E expression.