Objective:To detect the NJ001 antibody specific antigen expression in pancreatic cancer(PCa),and to investigate its relationship with clinicopathological features and prognosis value. Methods:The expression of NJ001 antibody specific antigen was evaluated through immunohistochemical staining in 89 PCa and 17 benign pancreatic disease. The results were scored by semiquantitative analysis. We analyzed the correlation of the results with clinicopathological parameters,and statistical analysis was evaluated by chi-square test or Fisher exact test. We also assessed the prognosis value using Kaplan-Meier survival curves. Results:The expression of this specific antigen was significantly higher in PCa tissues compared with benign pancreatic diseases(77.5% vs 17.6%,P < 0.001). We further found that the positive rate of NJ001 specific antigen in poor differentiation PCa patients was higher than that of well differentiation patients(83.0% vs 60.0%,P = 0.027). Meanwhile,the positive rate in patients with deep depth of tumor invasion or lymph nodes metastasis was higher than that of low invasion cases or no metastasis cases,respectively(82.2% vs 53.3%,P = 0.037;91.1% vs 62.8%,P = 0.002). Overall survival for patients with positive expression of the specific antigen was lower than that with negative expression(P= 0.036). Conclusion:The NJ001 antibody specific antigen expression in PCa was associated with differentiation,depth of invasion,lymph node metastasis and prognosis,suggesting the antigen may be a promising tumor marker for monitoring the development of Pca.