Objective:To study the effects of adenovirus mediated prostate apoptosis response-4 (Par-4)combined with cisplatin(CDDP)on subcutaneous xenografts of hunan Wilms’ tumor cells SK-NEP-1 in nude mouse. Methods:Inhibition effect of subcutaneous transplanted Wilms’ tumor model were established in BALB/c nude mice,and then the nude mice were randomly divided into five groups,namely Par-4+CDDP group,Par-4 group,CDDP group,negative control Par-4 group and blank control group. The volumes and weights of the transplanted tumors were measured. Transplanted tumor tissues were used to detect the expression levels of Par-4,GRP78 and BAX protein by HE staining and immunohistochemical analysis. Results:The tumor growth was inhibited in Par-4+CDDP group,Par-4 group and CDDP group,and there were differences in the tumor weight amorg 3 groups (P < 0.05). The inhibition effect of Par-4+CDDP group was superior to the Par-4 group and CDDP group. There was no statistically significace in tumor inhibition between blank control group and negative control Par-4 group. Par-4+CDDP can significantly increased Par-4,GRP78 and BAX protein(P < 0.05). Conclusion: Ectopic Par-4 in combination with CDDP suppressed the cancer-xenografted growth in nude mice,which might up-regulate BAX protein,the result of the interraction of extracellular Par-4 and GRP78.