Objective:To determine target genes of transcription factors THAP1 for a new therapeutic strategy of neonatal lung injury. Methods:Chromatin DNA in alveolar type Ⅱ epithelial cells was immunoprecipitated with THAP1 antibody. The obtained DNA samples were then subjected to high-throughput sequencing and bioinformatic analysis. Results:A total 20 417 enrichment peaks corresponding 9 688 genes was found. The mapping of the peaks revealed that the targeted nucleotide sequences located mainly within intergenic regions,intron regions and promoter regions,which contained 1 051 gene promoter regions. Gene Ontology(GO)analysis suggested that THAP1 regulated a set of genes,which were involved in various biological processes including protein dimerization activity,identical protein binding,protein phosphatase regulator activity,steroid hormone receptor activity. Signal pathway analysis showed that these genes were associated with endocytosis,glycan degradation,and biosynthesis-chondroitin sulfate/dermatan sulfate. Conclusion:The targeted genes of the transcription factor THAP1 in the alveolar type Ⅱ epithelial cells were determined. These results show that THAP1 is widely involved in various biological processes,which might provide a useful insight to reveal the function and mechanism of THAP1 gene on neonatal lung injury.