Objective:To observe the effect of stromal cell derived factor-1 (SDF-1)on human microvascular endothelial cell line-1(HMEC-1),and study the relevant mechanism. Methods:HMEC-1 cells were treated with SDF-1 of different concentrations(0,25,50,100 -滋g/L),then the phosphorylation level of PI3K/Akt and MAPK/Erk were detected by Western blot,and the proliferation ability and apoptosis rate of HMEC-1 cells were detected by MTT and FCM. Changes on migration ability of HMEC-1 cells was detected by wound healing assay. MCF-7 cells were pretreated with PI3K/Akt and MAPK/Erk signaling pathway inhibitor to observe the effect of PI3K/Akt and MAPK/Erk signaling pathway on SDF-1 induced proliferation,apoptosis and migration in HMEC-1 cells. Results:Compared with 0 -滋g/L SDF-1 treated group,25,50 and 100 -滋g/L SDF-1 treatment actived PI3K/Akt and MAPK/Erk signaling pathway,promoted proliferation and migration ability,and induced HMEC-1 cells apoptosis(P < 0.01),and those effects were concentration-dependent. When pretreated with PI3K/Akt and MAPK/Erk signaling pathway inhibitor,the effect of SDF-1 on promoting proliferation and migration ability,and inducing HMEC-1 cells apoptosis ability were significantly blocked. Conclusion:By activation PI3K/Akt and MAPK/Erk signaling pathway,SDF-1 can promote proliferation and migration ability,and induce HMEC-1 cells apoptosis,thus plays a role in diabetic angiopathy.