Objective:To analyze twin clinical reproduction outcome after in vitro fertilization (IVF)/intracytoplasmic sperm injection(ICSI)-embryo transfer(ET), the effective measure was explored in order to reduced ART multiple pregnancy rate. Methods:The data of 425 infertile couples were restrospectively analyzed,including pregnancy rate,pregnancy complications,delivery way and perinatal outcomes. Results:①There were IVF/ICSI-ET 4 603 cycles,the twin pregnancy rate was 23.35%,the abortion rate was 9.18%,the pregnancy complications occurrence rate was 13.41%,and the birth defects rate was 1.60%.②Between the two live born infant group and one live born infant group,there were statistically significant differences in fetal age[(29.66 ± 3.64) vs. (30.69 ± 3.47)week],birth weight[(2.64 ±0.45) vs. (3.12 ± 0.64)kg], cesarean delivery rate (97.68% vs. 77.38%),premature delivery rate (41.06% vs. 16.67%),low-weight baby occurrence rate(42.05% vs. 19.05)%. ③ Comparison of clinical data in mutifetal pregnancy reduction group and spontaneous reduction group with no reduction group,there were statistically significant differences in the fetal age[(38.00 ± 2.03),(37.89 ± 2.46) vs. (36.43 ± 1.77)week],birth weight [(3.05 ± 0.65),(3.15 ± 0.63) vs. (2.64 ± 0.45)kg] and premature delivery rate(13.64%,15.71% vs 37.24%). Conclusion:Compared the ART clinical reproduction outcome of twin with single live born infant,there were higher hazard in premature and lower birth weight. Women with ART pregnancies should get more perinatal care and antenatal examination. Clinical attention should be paid to diagnosis earlier,and multiple pregnancy reduction termination in time is very important for improving the perinatal outcomes.