Objective:To study the effect of isoliquiritigenin on the proliferation of human pancreatic cancer SW1990 cells and survivin expression. Methods: SW1990 cells were treated with different concentrations of isoliquiritigenin and then the cell proliferation was analyzed by MTT. Survivin mRNA and protein expressions were detected with Real-time PCR and Western blot, respectively. Results: In a certain concentration range, isoliquiritigenin significantly inhibited the proliferation of SW1990 cells in a dose- and time-dependent manner. Real-time PCR revealed that isoliquiritigenin down-regulated mRNA expression of survivin in SW1990 cells. The protein expression of survivin was also decreased by isoliquiritigenin treatment. Conclusion: Isoliquiritigenin can inhibit SW1990 cells proliferation and down-regulate survivin expression.