Objective:To study the role of resveratrol in regulating the browning of mouse primary white adipocytes and find out the related molecular mechanisms. Methods:Primary subcutaneous preadipocytes were isolated from the groin of C57BL/5J mice and induced for differentiation. The cells were treated with different concentrations(0-50 -滋mol/L) of resveratrol to analyze the resveratrol′s effect on the browning of white adipocytes. Real-time PCR was performed to detect the mRNA expression of BAT-specific genes and miR-27. mimic-miR-27b was transfected into primary cells to increase miR-27b expression,then,resveratrol (50 μmol/L)was performed to stimulate these cells. We used real-time PCR and Western blot to measure mRNA and protein levels of BAT-specific genes in these cells. Results:Resveratrol promoted the expression of BAT-specific genes and suppressed miR-27b expression in a concentration-dependent manner in mouse white adipocytes. Overexpression of miR-27b significantly inhibited the resveratrol action on the mRNA expression of BAT-specific genes. At the protein level,overexpression of miR-27b also suppressed the resveratrol action on the PRDM16 and UCP1 protein levels in mouse white adipocytes. Conclusion:Our data showed that resveratrol promoted the browning of mouse white adipocytes via suppressing miR-27b expression. MiR-27b might provide a potential therapeutic target for the treatment of obesity and metabolic syndrome.