Objective:To construct heart-specific lncRNA-uc.167 transgenic mice and analyze their phenotype. Methods:After double enzyme digestion by Sfi1 and Asc1,Vector plasmid pRP. ExSi Isl1 and purpose fragment uc167-pMD18■ Tplasmid were recycled and connected. And then, the uc.167 gene was cloned into the downstream of the Isl promoter specifically expressed in myocardial tissue to construct the transgene expression vector. Finally,we made the resulting vector linearization,established uc.167 transgenic mice by micro injection and detected the genotype by PCR. The phenotype of transgenic mice was preliminarily analyzed by cardiac histology and echocardiography. Results:We successfully constructed uc.167 transgenic mice. Echocardiography displayed that transgenic mice had no obvious abnormality in cardiac structure and function compared with the wild type mice. Heart weight index was not significantly changed both in positive and negative transgenic mice. HE staining suggested ventricular size and ventricular wall thickness had no significant difference and there were also no abnormal phenomenon including the change of cell size,inflammatory infiltration and cell edema in myocardial cell. Masson staining showed that both positive and negative transgenic mice had no significant change in myocardial fibrosis. Conclusion:Uc.167 transgenic mice did not appear obvious cardiac malformations,and cardiac structure and function were not significantly abnormal.