Objective:To investigate the new method of curing the type Ⅰ Diabetes by cultivating and trans-planting the composite artificial scaffold of MIN-6 β cells. Methods:Silk-collagen type Ⅳ scaffold was prepared by the method of freeze-drying. We implant MIN-6 β cells on the silk-collagen type Ⅳ scaffold and then observe the distribution situation of the growing cells and the vitality differences between those cells that growing on the ordinary plane and the composite scaffold. Reaction difference between those cells was compared. After transplantation,the change of the blood glucose level was monitored and the function of cell secretion was tested. Results:More β structure appeared after the silk- collagen type Ⅳ scaffold cross-linked by the methanol. The vitality of those cells cultivated on the scaffold was obviously better than those on the ordinary plane (P < 0.05). Both groups had a good stimulation reaction on both low & high concentration glucose,and the scaffold groups had a better cell secretory function than that of the ordinary plane groups(P < 0.05). MIN-6 β cells were dyed to brown and the cells still had a nice insulin secretory function. Conclusion:MIN-6 β cells which are three-dimensionally cultivated by the silk-collagen type IV scaffold have better vitality and relatively long-lasting insulin secretory function.