Objective:To investigate the effect of estrogen on lipid metabolism and glucose. Methods:Fotry 7-week-old female Sprague-Dawley rats were randomly divided into 4 groups,the control group(Con),sham-operated group(sham),ovariectomy(OVX)group and OVX rats given high dose E2 treatment (OVX+ E2). After being weighted,each group of rats were given corresponding processing. Nine weeks later,the change of visceral fat weight,total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL),high-density lipoprotein(HDL) and glucose were measured. Results:Compared with the control group,the body weight and abdominal fat of OVX group increased significantly. There was a significant difference in the level of TG,TC and LDL between the control and OVX groups. But in the high dose E2-treated OVX group it showed no significant differences. Compared with sham operation group rats,fasting blood glucose was of no difference in OVX group,but existed impairing glucose tolerance. Conclusion:The estrogen level was negatively correlated with the body weight,lower estrogen induced abnormal lipid metabolism and insulin resistance. But this phenomenon can be reversed after treated with estrogen.