Objective:To analyze the expression profile variation of long non-coding RNA(lncRNA) in lung tissues of newborn mice with hyperoxia-induced bronchopulmonary dysplasia (BPD). Methods:We first successfully established a newborn mouse model of hyperoxia-induced BPD. We used lncRNA microarray technology to inspect differences of lncRNA expression profile between normal control lung and BPD lung,which helped us screen out the lncRNAs with differential expression after pretreatment of raw data. Results: Compared with the control group,there were 1 769 lncRNAs with differential expression which had more than 2-fold changes and significant differences (P < 0.05),of which 882 increased more than 2 times and 887 reduced more than 2 times;140 increased more than 5 times;71 reduced more than 5 times;28 increased more than 10 times and 2 reduced more than 10 times. Conclusion:lncRNA expression profile of hyperoxia-induced BPD was significantly changed in comparison with normal lung tissues. These lncRNAs with differential expression may be involved in the occurrence and development of BPD.