Expression of HRD-1 on umbilical vein endothelial cells stimulating from oxLDL and related clinical importance
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    Abstract:

    Objective:To explore the role of HRD1 in apoptosis and lipid deposition caused by oxLDL in human umbilical vein endothelial cells (HUVECs). Methods:HUVECs were incubated with 0 μg/ml oxLDL as control group,or with 50 μg/ml oxLDL as oxLDL group. Western blot was used to analyze expression of HRD1 protein. HRD1 expression in vascular tissue was detected by immunofluorescence analysis. HRD1 effects on cell apoptosis and lipids deposition of HUVEC was detected by western blot and oil red o staining analysis. Results:Expression of HRD1 protein decreased significantly in oxLDL group than that in control group. Meanwhile,HRD1 also expressed in the athrosclerostic plaques,and predominantly in the endothelial cells. oxLDL induced the significant increases in apoptosis and lipids deposition of HUVEC. While the HRD1 overexpression by the infection of Ad-HRD1 markedly inhibited the Cleaved PARP and Cleaved Caspase 3 expression and lipids deposition caused by oxLDL. Conclusion:HRD1 inhibited the apoptosis and lipids deposition of HUVEC induced by oxLDL.

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玄文颖,李红妍,杨 珣,王 峰,李庆国,苏东明. HRD1在oxLDL刺激的人脐静脉血管内皮细胞中的表达及意义[J].南京医科大学学报(自然科学版英文版),2016,(5):559-563.

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  • Received:February 03,2016
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  • Online: May 23,2016
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