Objective:To produce a full molecule human anti-c-Met antibody with genetic engineering, and analyze the biological activity of the antibody and observe its influence on proliferation, migration and invasion of nasopharyngeal carcinoma cells. Methods:We designed a primer for amplifying the variable region of anti-c-Met Fab antibody. VH and VL genes were cloned into pFUSE-CHIg-hG1 and pFUSE-CLIg-hκ expression vectors, respectively, and were both transfected into eukaryotic cells. The expression product was purified using protein A column. The identification and immunological characteristics of antibody were analyzed by enzyme-linked immune sorbent assay (ELISA), Western blotting assay and immunofluorescence assay. The inhibition function of the antibody in nasopharyngeal carcinoma cells was analyzed by CCK-8. The effects of the antibody on the invasion and migration of nasopharyngeal carcinoma cells was determined by wound-healing assay and Transwell invasion assay. Results:The results demonstrated that the full molecule human anti-c-Met antibody was successfully produced, and the antibody effectively recognized c-Met protein. In nasopharyngeal carcinoma cell experiment, CCK8 assay demonstrated that the antibody played a significant role in proliferation of CNE2 and HONE1. Wound-healing assay and Transwell invasion assay demonstrated that the antibody could suppressed the migration and invasion of CNE2 and HONE1. Conclusion:In this study, full molecule human anti-c-Met antibody was established successfully and has obvious neutralizing effect. The antibody is expected to become a candidate molecule for molecular targeted therapy of nasopharyngeal carcinoma.