Objective:To investigate the effects of insulin resistance on hepatic lipid metabolism. Methods:We used RPMI 1640 with different insulin concentrations to stimulate HepG2 cells,and cells were divided into the insulin resistance (IR) group and the control group. Oil red O staining was performed to observe the cell morphology and intracelluar lipid droplets. Triglyceride was detected with the triglyceride assay kit. The expression of acetyl-CoA carboxylase (ACC),liver-type fatty acid-binding protein (L-FABP),lipoprotein lipase(LPL),carnitine palmitoyltransferase1 (CPT1),microsomal triglyceride transfer protein(MTP) and transcription regulatory factors sterol regulatory element-binding protein-1c (SREBP-1c),and peroxisome proliferator activated receptor α (PPAR α) were detected by real-time PCR. Results:HepG2 cell model of insulin resistance was induced after 1×10-4 mol/L insulin stimulated cells for 36 h. The intracelluar lipid droplets and triglyceride content were increased in the IR group than in the control group. The mRNA expressions of ACC and SREBP-1c in the IR group were higher than in the control group,and L-FABP had no significant difference compared with the control group. The mRNA expressions of LPL,CPT1,MTP and PPARα in the IR group were lower than those in the control group. Conculsion:Insulin resistance could induce abnormal lipid accumulation in HepG2 cells via increasing lipid synthesis and reducing lipid oxidation process.