Objective:To investigate the role of PUF family genes [Pumilio1 (Pum1) and Pumilio2 (Pum2)] in female fertility and meiotic maturation. Methods: We deleted the Pum genes by breeding mice harboring loxP sites in Pum1 (Pum1F/F Pum2-/-) with DEAD box polypeptide 4 (Vasa) promoter-mediated Cre recombinase mice (VDKO). Meanwhile. we analyzed the phenotype of VDKO mice. Results: Fertility test showed that female fertility of VDKO mice was reduced, but the mice could still produce offspring. However, in vitro meiotic maturation of VDKO oocytes was not affected. Conclusion: Pum proteins were not essential for mouse folliculogenesis or oocyte development.