Objective:To investigate the expression and function of CCN1 in hepatic ischemia-reperfusion (I/R)injury in mice. Methods:Partial warm ischemia was produced in the left and middle hepatic lobes of mice for 60 min,followed by reperfusion. Expression of CCN1 was detected by real-time PCR and Western blot at timed points. In indicated experiments,recombinant CCN1 or saline was injected via tail vein 1 h before hepatic ischemia. Levels of alanine aminotransferase (ALT)and aspartate aminotransferase(AST)in blood were examined at 6 h of reperfusion. Pathological analysis was performed with liver sections after hematoxylin and eosin staining. mRNA levels of cytokines were detected by real-time PCR. Results:CCN1 expression is up-regulated in hepatic I/R. Serum ALT and AST levels were significantly increased after CCN1 treatment. The pathological alterations were more severe compared with saline group. Gene transcripts for proinflammatory cytokines in liver tissue were elevated significantly in the CCN1 group. Conclusion:Induction of CCN1 in liver I/R aggravates hepatic injury,possibly by promoting the expression of inflammatory factors.