Objective:To establish a stable PNPLA7-expressed human hepatoma cell line (Huh7) and observe the effect of stable overexpression of PNPLA7 on the lipid contents in the cell line. Methods: pCMV6-PNPLA7-Flag plasmid was constructed and transfected into Huh7 cells with lipofectamine 2000 reagent. After G418 selection, mRNA and protein expression levels of PNPLA7 were examined by Western blot in these stable cell lines. Treated cells with oleic acid and the lipid contents in these cell lines were observed by oil red O staining. Results: Two lines stably expressing PNPLA7 were gained,and showed high expression level of PNPLA7 by Western blot analysis. The decreased lipid contents were observed in these cell lines after oleic acid treatment by oil red O staining. Conclusion: Huh7 cell lines stably expressing PNPLA7- Flag were established successfully. Stable overexpression of PNPLA7 may lead to the decreased lipid content in Huh7 cells after oleic acid treatment. It can be a reliable cell model for the study of functions and mechanisms of PNPLA7 in lipid metabolic pathways.