Objective:To establish the STO cell lines expressing the recombinant porcine leukemia inhibitory factor(LIF)and try to culture the rat induced pluripotency stem cells(riPSCs)with the established STO-pLIF cells as the feeder layer. Methods:We established the stable STO cell lines(STO-pLIF cells) which express LIF gene effectively by lipofectin transfection with the pig LIF eukaryotic expression vector pCAG-pLIF. The expression level of the inserted exogenous LIF gene was tested by RT-PCR,qPCR and Western blot. The cell lines which expressed pig LIF efficiently were used as the feeder cells to culture the riPSCs. Besides the RT-PCR testing the pluripotency factors,we also undertook the growth curve and the alkaline phosphates expressions detection as well as the immunocytofluorescent to identify the pluripotency of the riPSCs. Results: The expression level of the inserted exogenous LIF gene of the STO-pLIF cell lines was higher than that of the STO (P < 0.05), and the riPSCs cultured with the STO-pLIF were close to the traditional riPSCs, and had difference with the riPSCs cultured with the N2B27 without LIF (P < 0.05).Conclusion:The stable STO cell lines effectively expressing porcine recombinant porcine leukemia inhibitory factor were established successfully and these cells can maintain the pluripotency and self renew of riPSCs as the feeder cells.