Objective:To investigate the role of PEA3 in albumin induced mouse proximal tubular cells(mPTCs)apoptosis and its underlying mechanism. Methods:The cells were cultured with albumin in vitro,and divided into the control group,the vehicle control group,the PEA3 overexpression group,the albumin model group and the PEA3 overexpression in albumin model group. Real-time fluorescence quantitative polymerase chain reaction was used to investigate the level of PEA3 gene transfection efficiency. Flow cytometry and Hoechst staining technique were performed to analyze the apoptosis of mPTCs. The mRNA and protein levels of E-cadherin,α-SMA and Vimentin were detected by RT-PCR and Western bolt. Results:Transfection of PEA3 over-expresed the mRNA level of PEA3 for 2.5 fold. The apoptosis of mPTCs treated with albumin was increased compared with the control group,and overexpression of PEA3 ameliorated albumin-induced apoptosis. Albumin treatment reduced the expression of E-cadherin and increased the expression of Vimentin and α-SMA. Overexpression of PEA3 reversed the reduction of E-cadherin and ameliorated the increase of Vimentin and α-SMA. Conclusion:Overexpression of PEA3 can protect mPTCs from apoptosis and epithelial-mesenchymal transition induced by albumin.