Objective: To examine the expression of crystallin-alpha B (CRYAB) in human laryngeal squamous cell carcinoma(LSCC) and investigate the relationship between its expression and clinical characteristics of LSCC. Methods: One-step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) test in 10 fresh LSCCs as well as non-cancerous tissue samples and immunohistochemistry analysis by tissue microarrays (80 LSCC samples and 20 tumor-adjacent normal samples) were performed to characterize CRYAB expression in LSCC. Cox regression and Kaplan-Meier survival analyses were carried out to evaluate the prognosis of LSCC. Results: The results of qPCR test and immunohistochemistry analysis showed that the expression of CRYAB in LSCC was significantly higher than that in tumor-adjacent normal tissues(P = 0.0005 and P = 0.021, respectively). Moreover, the expression level of CRYAB protein in LSCC was significantly related to tumor differentiation(P = 0.022), TNM stage(P = 0.013),lymph node metastasis (P = 0.026) and overall survival (P = 0.001). COX multi-factor analysis showed that CRYAB expression (P = 0.036) was recognized as an independent prognosis factors for LSCC. Conclusion: The data suggest that CRYAB expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.