Objective:To explore an intrathecal catheterization method that suitable for T10 spinal cord injury(SCI) rats model,which can be better used for dosing subarachnoid space in (spinal cord injury,SCI) model. Methods:A total of 60 female SD rats were randomly divided into the L3~4 catheterization group(T group) and the L6~S1 catheterization group(C group). After 7 days of intrathecal catheterization and SCI,the differences between two types of intrathecal catheterization in disability rate,mortality,biting catheter rate,lidocaine test,weight changes and blue ink location test were compared. After 7 days of SCI,Alzet osmotic pumps were implanted into two groups respectively,then we observed the mortality,weight and the morphology of the spinal cord after long-term catheter and osmotic pumps implanted in SCI rats. Results:After 7 days of intrathecal catheterization and SCI,the biting catheter rate of the two groups had significant difference (P < 0.05),and total elimination rate had significant difference (P < 0.01). After intrathecal catheterization and SCI,the weights of the two groups were decreased,and after 35 days of intrathecal catheterization,the weight of rats in the T group was higher than that in the C group(P < 0.05). Blue ink location test showed that there was no appearance of catheter obstruction and position migration in the two groups. After 4 weeks of Alzet osmotic pumps implanted,the histocompatibility of Alzet osmotic pumps were well. HE staining showed that the lumbar spinal cords in the two groups were all been compressed mildly. Conclusion:The L3~4 intrathecal catheterization is ideal for SCI model in rats as the biting catheter rate and mortality is lower,and the operation is easier.